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Blood test for men

Testosterone test: total and free testosterone in one blood test.

One testosterone number says little, because by far the largest share is bound to SHBG. This test measures 9 hormone values in one draw: how much testosterone is actually available, and where a low value comes from.

No referral needed · €129 one-time
9
hormone values in the testosterone test
250+
draw locations in the Netherlands
<24h
results, in plain language
4.8 out of 5 stars from 80 reviews after the blood test
Analysis by accredited labs in the Netherlands
01 Inside

Which hormone values does a testosterone test measure?

Eight values that together give the whole picture. Total testosterone is the starting point, but by far the largest share of it is bound to SHBG and albumin. Free testosterone and the free androgen index show what is actually available to your muscles, your energy and your libido.

LH and FSH tell you where a low value comes from: the testes themselves, or the signalling from the brain. Prolactin and estradiol complete the picture. Elevated prolactin can lower your testosterone, and estradiol shows how much testosterone is converted into estrogen. Every value comes with a plain-language explanation in the app and has its own page in the biomarker library.

02 Threshold

When is your testosterone too low?

There is no national threshold. Four Dutch labs, four lower limits: for men up to 50 the lower limit for total testosterone runs from 6.1 to 9.0 nmol/L, and at the three labs that publish free testosterone from 165 to 210 pmol/L. The same value is low at one lab and normal at another. That is why the app shows your value against the reference ranges of the lab that ran your sample, and the total testosterone page puts the labs' tables side by side.

Guidelines only speak of a deficiency when a low value has been measured twice in the morning and there are symptoms to go with it. One low value is a reason to repeat, not a conclusion. Which symptoms those are is explained under low testosterone.

03 Practical

What time should you draw for a reliable testosterone value?

Testosterone peaks in the morning and drops over the day. So book your draw early, preferably before 10:00. Every draw is fasted. Reference ranges and guidelines assume a morning measurement, which makes your value comparable with the lab and with your next test.

Slept badly or just been ill? That lowers your testosterone temporarily. Draw on an ordinary day, so you measure yourself and not your week.

With Optimize you book the appointment yourself at one of the 250+ draw locations, without a doctor's referral. Your 9 values are typically in the Optimize app within 24 hours, with an explanation per hormone and how they relate.

Markers explained

What does each value in the testosterone test measure?

Eight hormone values, each with its own role in the picture of your testosterone, plus one optional add-on: Prostate health (PSA).

Total testosterone
Total testosterone adds up all the testosterone in your blood, the part bound to protein and the free part together. It is the hormone that carries muscle, bone, desire and energy, and one of the hormones that move most with how you live. Have blood drawn before ten in the morning, and read it beside SHBG, which decides how much of it works.
Free testosterone
Free testosterone is the part of your testosterone not bound to protein and therefore able to do its work, calculated from your total testosterone, your SHBG and your albumin. It is the number that fits complaints best when SHBG is abnormal.
FAI (free androgen index)
The free androgen index is a calculation: your total testosterone divided by your SHBG, the protein that binds testosterone. The result estimates how much of your testosterone is free and therefore works, and is above all in women with excess hair growth, acne or an irregular cycle a more usable number than the total alone.
SHBG
SHBG, in full sex hormone binding globulin, is the protein from your liver that holds testosterone and oestradiol in your blood and so decides how much of them stays active. It is also one of the first values to give away insulin resistance. Read it beside total testosterone and have the free share calculated.
LH
LH, in full luteinising hormone, is the hormone from your pituitary that in women gives the starting signal for ovulation and in men sets the testes to making testosterone. In women not one normal value but a range per cycle phase, with a surge around ovulation a multiple higher than the rest.
FSH
FSH, in full follicle-stimulating hormone, is the signal with which your brain sets the ovaries or the testes to work: low when they respond well, high when they respond less, and permanently high after the menopause. In women not one normal value but a range per cycle phase.
Prolactin
Prolactin is the hormone from your pituitary that starts milk production after childbirth and outside that time acts as a brake on your sex hormones: a raised value suppresses LH and FSH, and with them the cycle, desire and testosterone. Have blood drawn in the morning, rested and without exercise or sex beforehand, and read it beside LH, FSH and TSH.
Oestradiol
Oestradiol is the main oestrogen: in women the hormone that carries the first half of the cycle and keeps bone, vessels and mucous membranes healthy, in men a small amount that arises from testosterone. A regular cycle with ovulation is the health signal, and eating too little, training too much and stress are what switch it off.
PSA
PSA, in full prostate-specific antigen, is a protein made almost entirely by the prostate, a small amount of which reaches your blood; on your results it is also called tPSA or total PSA. It is specific to the prostate, not to prostate cancer, and the series over the years says more than one number.

add-on

Ready to get your testosterone tested? Order the Hormones men test on its own, or add it as an add-on to the comprehensive test or to your .

Test your hormones
Single testosterone value vs Optimize

What you get that a single testosterone value misses.

Total testosterone
Free testosterone
Free androgen index (FAI)
SHBG
LH and FSH (testes or signalling)
Prolactin
Estradiol
Per-marker explanation
Every marker
Trend over time
Built in

Clarity on your testosterone and what drives it

Single Hormones men test: 9 values, no membership needed
Total and free testosterone, with SHBG, LH, FSH, prolactin and estradiol
Track your values over the years
Total testosteroneFree testosteroneFree androgen indexSHBGLHFSHProlactinEstradiol (E2)Total testosteroneFree testosteroneFree androgen indexSHBGLHFSHProlactinEstradiol (E2)Total testosteroneFree testosteroneFree androgen indexSHBGLHFSHProlactinEstradiol (E2)
Estradiol (E2)ProlactinFSHLHSHBGFree androgen indexFree testosteroneTotal testosteroneEstradiol (E2)ProlactinFSHLHSHBGFree androgen indexFree testosteroneTotal testosteroneEstradiol (E2)ProlactinFSHLHSHBGFree androgen indexFree testosteroneTotal testosterone

How to get your testosterone tested

Order the Hormones men test

9 hormone values in one draw, €129 one-time. Checkout takes two minutes, no referral needed.
CHOOSE YOUR PATH
Hormones men€129 one-time
Core membership€365 a year

Want more than your hormones?

Then add the test as an add-on to the comprehensive test with 52 values: €109 extra, in the same draw. Or pick the (€1 a day) and add it to your annual blood test.
CHOOSE YOUR TEST
Hormones men€129 one-time
Comprehensive test+€109 add-on

Book your draw in the app

In the Optimize app, book an appointment at one of the 250+ draw locations. Pick a morning slot, preferably before 10:00, and come fasted.
DRAW DAY
Fasted from midnight
WATER IS FINE
Blood draw
THU 18 SEP · 08:15 · 3 MIN

Understand your testosterone values

See where each hormone stands against the lab's reference ranges, with an explanation of what it means and how the values relate.
HORMONES MEN
Total testosterone
10.4nmol/L
Free testosterone
92pmol/L
SHBG
84nmol/L
LH
4.6U/L

Track change over time

Re-test after three months to see how your values move with sleep, weight and training.
IMPROVEMENTS
Free testosterone
LowNormal
SHBG
HighNormal
RE-TEST: 9 DEC 2026
FAQ

Frequently asked questions

The key things to know about testosterone testing with Optimize.

Yes. With Optimize you order the Hormones men test online and book an appointment at a draw location yourself, without a referral. You get total and free testosterone, SHBG, LH, FSH, prolactin and estradiol in one draw. Want to discuss your results with a doctor? They travel with you from the app.

Take control now.

Where to start

Hormones men

€129one-off

One draw at a location near you, results explained in the app.

  • 9 blood values in one draw
  • Results within 24 hours, in plain language
  • No referral, no waiting list
  • 250+ draw locations in the Netherlands
  • Your trends on every retest, in the app
Test your hormones

No referral needed · Results within 24 hours

Rather measure continuously? Core membership with Hormones men (€474 now, then €365 a year)

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