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Hormones

Total testosterone

Total testosterone adds up all the testosterone in your blood, the part bound to protein and the free part together. It is the hormone that carries muscle, bone, desire and energy, and one of the hormones that move most with how you live.

Medically reviewed by
Aäron Spapens
Aäron Spapens
Lifestyle physician at Optimize

In short

Normal in a man of 20 to 50 years is 9.0 to 27.3 nmol/L and in a woman 0.3 to 1.7 nmol/L, measured in the morning; testosterone moves strongly with belly fat, sleep, stress and alcohol, and in most men with a low number that is the reversible side.

  • A low value in a man is usually drawn too late, belly fat, lack of sleep, stress, alcohol or overtraining, and rarely the testes themselves; LH shows where it lies, and a low value is repeated on a second morning.
  • A high value in men is usually testosterone from outside; in women a raised testosterone usually belongs to PCOS, with insulin resistance underneath.
  • Have blood drawn before ten in the morning, and read the value beside SHBG and LH; with a high or low SHBG the total tells only half the story.
On this page7

What is total testosterone?

Testosterone is the main male sex hormone, but everyone has it. In men the testes make it, driven by LH from the pituitary; in women a tenth to a twentieth of that amount comes from the ovaries and the adrenal glands. It builds muscle and bone, drives desire, energy and drive, keeps the production of red blood cells up and, in men, helps decide sperm production. Most testosterone in your blood travels bound to protein: tightly to SHBG and loosely to albumin. Only the free part, a few percent, can enter the cell and do its work. Total testosterone adds everything up, bound and free. That is the standard measurement and usually enough, but with a high or low SHBG the active part shifts without the total moving, and then SHBG belongs beside it. Your report shows testosterone in nmol/L, with one range for men and one for women that do not meet: where the labs overlap it runs from 9.0 to 27.3 nmol/L in a man of 20 to 50 years and from 0.3 to 1.7 nmol/L in a woman of the same age. The range falls slightly with age, and it depends on the time of day: testosterone is highest in the morning and a quarter lower in the afternoon, so have blood drawn before ten.

Why is total testosterone relevant?

For men testosterone is one of the most honest lifestyle values on the report. It falls with belly fat, because fat tissue converts testosterone into oestradiol and insulin resistance suppresses production. It falls with lack of sleep, because most testosterone is made during sleep; a week of short nights already makes a noticeable difference. It falls with lasting stress, with plenty of alcohol, with overtraining and eating too little, and with sleep apnoea. And it falls slowly with age, about one percent a year from around thirty, though in fit men much less than assumed. That makes a low testosterone in a man of forty usually not a matter of a hormone that has run out, but of a body that does not make it because the conditions are not right. Little desire, little energy, less muscle and more belly are the complaints, and they reinforce each other: less testosterone gives more belly fat, and more belly fat gives less testosterone. Anyone who breaks that circle, with losing weight, strength training and sleep, usually sees the number come back. In women testosterone plays a different role: it carries desire, energy and muscle, and the most common problem is too much rather than too little. A raised testosterone in a woman usually belongs to PCOS, with an irregular cycle, excess hair growth and acne, and there too insulin resistance sits underneath. Testosterone as a medicine is there for men with a persistently low testosterone on two mornings and symptoms, once LH has shown where it lies. It is not a remedy for tiredness or getting older: it shuts down production of your own, reduces fertility and thickens the blood, and in most men with a moderately low number lifestyle works better and without that price.

Total testosterone reference ranges

What counts beside the number:

  • The time: before ten in the morning, after a normal night, and a low value repeated on a second morning
  • SHBG, which shows how much of the total is free; with excess weight or with age the total tells only half the story
  • LH, which with a low value shows whether it is the testes or the signal from the brain
  • Your waist, sleep, alcohol, training load and stress, the levers the number responds to
  • Medicines: opioids, prednisone, anabolic steroids, and in women the pill
  • In women: the cycle, excess hair growth and acne, which point at PCOS
Total testosterone reference ranges
GroupReference range
Men 20 to 50 yrsWhere the labs overlap, measured in the morning; the range on your own report may be wider, especially at the top9.0–27.3 nmol/L
Men from 50 yrsSlightly lower than the band above, because testosterone falls slowly with age; in fit men much less than assumed8.8–25.7 nmol/L
Women 20 to 50 yrsA tenth to a twentieth of the men's band; above the range usually belongs to PCOS0.3–1.7 nmol/L
Women from 50 yrsAfter the menopause the ovaries make less, and the range falls with it0.17–1.4 nmol/L

The bands above are where the labs overlap; the range on your own report may be wider, and there is no national normal value. The bands for men and women do not meet. All bands apply to a morning value; in the afternoon testosterone sits a quarter lower. With a high or low SHBG the total tells only half the story, and then free testosterone belongs beside it.

What Dutch labs actually use

The four labs publish two bands for testosterone that do not meet, one for men and one for women, with a gap in between. Where they overlap, the female band from 20 to 50 yrs stops at 1.7 nmol/L and the male band of the same age only starts at 9.0 nmol/L. For men the difference sits mainly in the ceiling. Unilabs runs its band up to 37.3 nmol/L and Clinical Diagnostics stops as low as 27.3, with Certe and Star-shl in between at 32.4 and 29.0. The floors sit closer together: 9.0 at Unilabs, 8.8 at Certe, 8.6 at Star-shl and 6.1 at Clinical Diagnostics. The second line is age, and the labs fill it in differently. Unilabs lowers the male band in two steps, around the fiftieth year and once more above eighty; Star-shl lowers it once, at 50; Certe and Clinical Diagnostics hold one band. In the shared band the male floor therefore drops from 9.0 down to 8.8 nmol/L, less than the labs differ from each other: their floors for a man from 20 to 50 yrs run from 6.1 to 9.0 nmol/L. Where the four labs overlap, a man from 20 to 50 yrs is left with 9.0 to 27.3 nmol/L and from 50 yrs with 8.8 to 25.7 nmol/L. A woman is left with 0.3 to 1.7 nmol/L from 20 to 50 yrs and with 0.17 to 1.4 nmol/L from 50 yrs.

Woman aged 35 · every lab calls this normal

0.3 – 1.7nmol/L

Between 0 and 0.3 and between 1.7 and 2.6 it depends on the lab, because each sets its limits on its own method and population. Above 2.6 every lab calls it abnormal.

Unilabs(Saltro, Medlon, SHO, Atalmedial)
0.2–2.1 nmol/L
0.3–1.7 nmol/L
0.17–1.81 nmol/L
0–2.6 nmol/L

every lab calls 0.3 – 1.7 nmol/L normal. Between 0 and 0.3 and between 1.7 and 2.6 it depends on the lab.

Every age, per lab
What Dutch labs actually use
LaboratoryGroupReference range
Unilabs(Saltro, Medlon, SHO, Atalmedial)2026Men 20 to 50 yrs9.0–37.3 nmol/L
Men 50 to 80 yrs7.6–37.1 nmol/L
Men from 80 yrs5.4–36.8 nmol/L
Women 20 to 50 yrs0.2–2.1 nmol/L
Women from 50 yrs0.1–1.8 nmol/L
Star-shl2026Men 18 to 49 yrs8.6–29.0 nmol/L
Men from 50 yrs6.7–25.7 nmol/L
Women 18 to 49 yrs0.3–1.7 nmol/L
Women from 50 yrs0.1–1.4 nmol/L
Certe2026Women 16 to 20 yrs0.1–1.7 nmol/L
Men 16 to 20 yrs4–24 nmol/L
Women (all ages)0.17–1.81 nmol/L
Men (all ages)8.8–32.4 nmol/L
Clinical Diagnostics2026Men6.1–27.3 nmol/L
Women0–2.6 nmol/L

Each band as the lab publishes it, retrieved in 2026, unless marked otherwise.

Total testosterone high or low: what it means

A low testosterone in a man is, in order of how often it occurs: drawn too late in the day or after a short night, belly fat and insulin resistance, lack of sleep or sleep apnoea, lasting stress, plenty of alcohol, overtraining or eating too little, and age. Then medicines: opioids, prednisone and anabolic steroids, which shut down production of your own. Rarely it is the testes themselves or the pituitary, and LH keeps those two apart: a high LH with a low testosterone is the testes, a low or normal LH is the signal from the brain, and that is almost always the reversible part. A high testosterone is rarely a problem in men and usually testosterone from outside, from a gel, injection or anabolic steroids. In women a raised value is usually PCOS; a strongly raised value, a multiple of the range, is rare and calls for investigation of ovaries and adrenal glands. A low testosterone in women is rarely measured and rarely treated: the pill lowers it via SHBG, and that is then the intention. The levers are strong and concrete in men. Losing weight around the middle is the biggest, and ten percent weight loss already lifts testosterone noticeably in a man with excess weight. Strength training, because muscle demands and makes testosterone, and heavy exercises with large muscle groups work best. Seven to eight hours of sleep, and having sleep apnoea treated if it is there. Less alcohol, and enough food for what you train: an energy deficit shuts the signal down. In women with PCOS, losing weight and less sugar and fast carbohydrates work the same way, through the insulin resistance underneath. Never read testosterone on its own. SHBG shows how much of the total is free; in a heavy or ageing body the total tells only half the story. LH shows where a low value comes from. In men oestradiol belongs beside it when there is belly fat, in women the cycle day. And repeat a low value on a second morning before drawing anything from it.

What lowers it

Usually the moment of the draw and the conditions you live in; rarely the testes themselves.

What lowers it: Total testosterone
CauseHow often
The time of the drawTestosterone is highest in the morning and a quarter lower in the afternoon; after a short night it also sits lower. Draw before ten and repeat a low value on a second morningOften
Excess weight and insulin resistanceBelly fat converts testosterone into oestradiol and insulin resistance suppresses production and SHBG; the largest reversible cause in menOften
Lack of sleep and sleep apnoeaMost testosterone is made during sleep; a week of short nights already makes a noticeable difference, and sleep apnoea suppresses it persistentlyOften
AgeAbout one percent a year from around thirty, much less in fit men; that is why the labs let the range fall slightlyOften
Eating too little, training too much or lasting stressAn energy deficit and cortisol turn the signal from the brain down low; LH and testosterone drop togetherSometimes
Medicines that shut production downOpioids, prednisone and anabolic steroids suppress LH and with it production of your own; after steroids recovery takes monthsSometimes
Testes making lessAfter an infection, from a congenital cause or after chemotherapy; LH then sits high instead, and that is the difference from all the causes aboveRare

What raises it

In men usually from outside, in women usually PCOS.

What raises it: Total testosterone
CauseHow often
Testosterone from outsideA gel, injection or anabolic steroids lift the total and shut down production of your own; LH and FSH then sit lowSometimes
Polycystic ovary syndrome (PCOS)In women the most common reason for a raised testosterone, with an irregular cycle, excess hair growth and acne; insulin resistance and belly fat sit underneathSometimes
A tumour of the ovary or adrenal glandRare; the value then sits a multiple above the range and the symptoms come on quicklyRare

How does a total testosterone blood test work?

Referral
Not needed. You can have testosterone measured without going through your GP.
When to draw
Before ten in the morning, after a normal night and not after a heavy training session or an evening with plenty of alcohol. In the afternoon the value sits a quarter lower.
Fasting
Preferably yes: a meal lowers testosterone temporarily. So have blood drawn before breakfast.
The draw
One tube of blood from a vein in your arm, with SHBG and LH from the same tube.
The result
A single number in nmol/L, with one range for men and one for women. Usually available within a few days.
Repeating
A low value always on a second morning, because testosterone fluctuates from day to day. After losing weight, strength training or sleeping better you see the difference after three to six months.
Where to draw
You can draw at 250+ locations near you, no referral needed. See the locations

Frequently asked questions

What is total testosterone?

All the testosterone in your blood together: the part bound to SHBG and albumin and the free part that can do its work in the cells. It is the hormone that carries muscle, bone, desire and energy, in men in large amounts and in women in small ones. With a high or low SHBG the active part shifts without the total moving, and then SHBG belongs beside it.

What is a normal testosterone level?

In a man of 20 to 50 years 9.0 to 27.3 nmol/L and from 50 years 8.8 to 25.7 nmol/L, measured in the morning. In a woman of 20 to 50 years 0.3 to 1.7 nmol/L and from 50 years 0.17 to 1.4 nmol/L. The range on your own report may be wider. For men what counts beside the number is mainly how it relates to your waist, sleep and complaints: a value at the bottom of the range in a man with belly fat and little energy is an invitation, not a diagnosis.

What does a low testosterone mean?

In a man usually that the conditions are not right: drawn too late in the day, belly fat and insulin resistance, lack of sleep or sleep apnoea, stress, plenty of alcohol, overtraining or eating too little. Those lower the signal from the brain, and LH then sits low or normal. If LH sits high instead, it is the testes themselves, and that is rare. Repeat a low value on a second morning before drawing anything from it.

What does a high testosterone mean?

In men rarely a problem and usually testosterone from outside, from a gel, injection or anabolic steroids; LH and FSH then sit low because production of your own has stopped. In women a raised testosterone usually belongs to PCOS, with an irregular cycle, excess hair growth and acne. A value a multiple above the range is rare and calls for investigation.

How do I raise my testosterone naturally?

Losing weight around the middle is the biggest lever: ten percent weight loss already lifts testosterone noticeably in a man with excess weight. Strength training with heavy exercises for large muscle groups. Seven to eight hours of sleep, and having sleep apnoea treated. Less alcohol. Enough food for what you train, because an energy deficit shuts the signal down. Count on three to six months before you see it back in the number.

When is testosterone as a medicine appropriate?

In men with a persistently low testosterone on two mornings and symptoms, once LH has shown where it lies and the reversible causes have been addressed. It is not a remedy for tiredness or getting older: it shuts down production of your own and fertility and thickens the blood. With a moderately low number lifestyle works better in most men and without that price.

Why is testosterone drawn in the morning?

Because it follows a daily rhythm: highest in the early morning, a quarter lower in the afternoon. All ranges apply to a morning value. Have blood drawn before ten, after a normal night, preferably before breakfast, and not after a heavy training session or an evening with plenty of alcohol. Because testosterone also fluctuates from day to day, a low value is repeated on a second morning.

Do I have to fast for a testosterone test?

Preferably yes: a meal lowers testosterone temporarily, so drawing before breakfast gives the cleanest value. More important still is the time, before ten in the morning. Have SHBG and LH taken from the same tube, because without those two a low value cannot be placed.

Which tests include total testosterone?

Total testosterone is in the Hormones men test (€129, also as an add-on for €109).

Related biomarkers

Read on

Sources

  1. 1.Unilabs, test catalogue (Dutch): total testosterone. 2026. bepalingenklapper.nl
  2. 2.Star-shl, test catalogue (Dutch): total testosterone. 2026. star-shl.nl
  3. 3.Certe, test catalogue (Dutch): total testosterone. 2026. bepalingenwijzer.certe.nl
  4. 4.Clinical Diagnostics, lab guide (Dutch): total testosterone. 2026. clinicaldiagnostics.nl

Educational information only, not medical advice. Consult a healthcare professional for clinical decisions.

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