How is my biological age calculated?

Optimize uses PhenoAge, a scientifically validated formula that calculates biological age from nine biomarkers plus your chronological age. The biological age test measures everything the formula needs in one draw, and your PhenoAge score appears in the app.

What does the biological age test cost?

A standalone biological age test costs €69, from a single blood draw. And the Core membership (€1 a day) includes it, recalculated every year.

Can I lower my biological age?

Lifestyle has significant influence on many of the markers PhenoAge uses, such as inflammation (CRP), blood sugar (glucose) and immune markers (lymphocytes, white blood cells). Improvements in those markers lower your calculated biological age in re-tests.

Is this an epigenetic or telomere test?

No. Optimize calculates your biological age with PhenoAge: a formula on nine blood values and your calendar age, from an ordinary blood test. No DNA methylation and no telomeres, but a score you get again at every re-test and can therefore track.

Do I need a referral from my doctor?

No. You can request the blood test yourself, without a referral.

Blood test

Biological age test: one score from your blood.

Name: Biological age test: one score from your blood.
Creator: Optimize

Your calendar age counts your years. Your biological age says how your body is doing. PhenoAge turns nine blood values and your age into that score: as a single test, or every year again in your .

Test your biological age

No referral needed · €69 one-time

or : these 19 values plus 41 more, every year

in the PhenoAge formula

250+

draw locations in the Netherlands

<24h

results, in plain language

4.8 out of 5 stars from 80 reviews after the blood test

Analysis by accredited labs in the Netherlands

01 Concept

What is biological age?

Your calendar age is a fixed number. Your biological age is not. It is an estimate of how old your body is physiologically, based on measurable markers in your blood. Two people aged 40 can have biological ages of 32 and 48, depending on what their numbers say.

Not a score to judge yourself against, but a way to see what your lifestyle is actually doing. A change in sleep, nutrition, training or stress shows up in markers tied, directly or indirectly, to ageing.

02 Calculation

How exactly is your biological age calculated?

Optimize uses PhenoAge, a method developed by Morgan Levine and colleagues (2018), built on almost 10,000 participants of the US NHANES III study and validated in an independent NHANES cohort. The calculation runs in two steps.

03 Progress

Build your trend

A single measurement gives you a starting point. The value is in repetition: re-testing every 6 to 12 months shows whether the changes you make (less ultra-processed food, more strength or endurance training, better sleep) actually lower your biological age.

That way biological age stops being a one-off score and becomes a direction you can verify.

Ready to calculate your biological age? Order the test on its own, or start a , where it comes with every annual test.

Test your biological age

The PhenoAge markers behind your biological age

The nine biomarkers PhenoAge uses, grouped by category. Click a marker to learn why it was chosen.

Immune system

3 biomarkers

Oxygen transport

2 biomarkers

Organs

3 biomarkers

Nutrients & metabolism

1 biomarker

Glucose (fasting)

Nine markers. One score that reveals your real age.

Calculate your biological age via PhenoAge

As a single test, or every year again in the Core membership

See whether your age moves with your lifestyle

AlbuminCreatinineGlucose (fasting)C-reactive protein (CRP)Lymphocyte %MCVRDWAlkaline phosphataseLeukocytesAlbuminCreatinineGlucose (fasting)C-reactive protein (CRP)Lymphocyte %MCVRDWAlkaline phosphataseLeukocytesAlbuminCreatinineGlucose (fasting)C-reactive protein (CRP)Lymphocyte %MCVRDWAlkaline phosphataseLeukocytes

LeukocytesAlkaline phosphataseRDWMCVLymphocyte %C-reactive protein (CRP)Glucose (fasting)CreatinineAlbuminLeukocytesAlkaline phosphataseRDWMCVLymphocyte %C-reactive protein (CRP)Glucose (fasting)CreatinineAlbuminLeukocytesAlkaline phosphataseRDWMCVLymphocyte %C-reactive protein (CRP)Glucose (fasting)CreatinineAlbumin

How to calculate your biological age

Order the biological age test

Nineteen values in one draw, €69 one-time, no referral. Checkout takes two minutes.

CHOOSE YOUR PATH

Biological age€69 one-time

Core membership€365 a year

Rather every year again?

The (€1 a day) includes your annual blood test with all the PhenoAge markers, so your score is recalculated every year and you see the trend.

CHOOSE YOUR PATH

Biological age€69 one-time

Core membership€365 a year

Book your draw in the app

In the Optimize app, book an appointment at one of the 250+ draw locations near you.

DRAW DAY

Fasted from midnight

WATER IS FINE

Blood draw

THU 18 SEP · 08:15 · 3 MIN

Receive your biological age

Your PhenoAge score appears in the app, alongside the individual markers it is based on.

BIOLOGICAL AGE

33.4PHENOAGE+2.4 years

LAST TEST: 9 SEP 2026

Track change over time

Re-test after 6 to 12 months to see whether your biological age moves with the changes you make.

PROGRESS

28.4PHENOAGE−2.6 years

RE-TEST: 4 MAR 2027

FAQ

Frequently asked questions

The key things to know about measuring biological age with Optimize.

Biological age is an estimate of how old your body is physiologically, based on measurable biomarkers in your blood. It can differ from your calendar age, higher or lower, depending on lifestyle, genetics and health.

Take control now.

Where to start

Biological age

€69one-off

One draw at a location near you, results explained in the app.

19 blood values in one draw
Results within 24 hours, in plain language
No referral, no waiting list
250+ draw locations in the Netherlands
Your trends on every retest, in the app

Test your biological age

No referral needed · Results within 24 hours

Rather measure continuously? Core membership (€1 a day)

References

[1]Levine ME, Lu AT, Quach A, et al. An epigenetic biomarker of aging for lifespan and healthspan. Aging Cell. 2018;17(4):e12816. https://doi.org/10.1111/acel.12816
[2]Levine ME. Modeling the rate of senescence: can estimated biological age predict mortality more accurately than chronological age? J Gerontol A Biol Sci Med Sci. 2013;68(6):667-674. https://doi.org/10.1093/gerona/gls233
[3]National Center for Health Statistics. National Health and Nutrition Examination Survey III (NHANES III), 1988-1994. Centers for Disease Control and Prevention. https://www.cdc.gov/nchs/nhanes/nhanes3/nhanes3.htm